TRYPSIN-BOVINE USP
T-82848
DESCRIPTION:
T82848 is a white powder manufactured from bovine pancreas collected from BSE, TSE free countries currently on the European Unions free countries list. The materail is manufactured to meet or exceed USP standards During the manufacture the Trypsin is subjected to pH of <2 and further purified.
APPLICATIONS:
- Used extensively in protein sequencing
- Tissue culture work
- Tissue dissociation
- Microbiological media
| ANALYSIS | SPECIFICATION | TYPICAL |
| ASH (RESIDUE UPON IGNITION) | Maximum 2.5% | 0.88% |
| MOISTURE (LOSS ON DRYING) | Maximum 5% | 2.5 % |
| AEROBIC COUNT | <100 organisms/gram | 00.0 organisms/gram |
| PSEUDOMONUS AERUGINOSA | Not Detected | Not Detected |
| E. COLI | Not Detected | Not Detected |
| SALMONELLA | Not Detected | Not Detected |
| STAPHYLOCOCCUS AUREUS | Not Detected | Not Detected |
| ASSOCIATED ACTIVITY | Report Chymotrypsin | Report Chymotrypsin |
| TRYPSIN ACTIVITY | >2500 USP units/mg | 2900 USP units/mg |
| SOLUBILITY | 500,000 USP Trypsin units In 10 ml water |
Clear-buff solution |
| SOLUBILITY | 500,000 USP Trypsin units In 10 ml saline |
Clear-buff solution |
| STORAGE: | 2-8C |
CHYMOTRYPSIN: THE AMOUNT OF ENZYME CAUSING A DECREASE IN ABSORBANCE of 0.0075 PER MINIUTE AT 25C.
TRYPSIN: THE AMOUNT OF ENZYME CAUSING A DECREASE IN ABSORBANCE of 0.003 PER MINIUTE AT 25C.
METHOD OF ASSAY: SCHWERT,G.W. AND TAKENAKA.