TRYPSIN-PORCINE
T-77

DESCRIPTION:
Trypsin T-77 is a proteolytic enzyme that is a off white to beige colored powder. T-77 is extracted from USDA certified porcine pancreas glands. It is classified as a Serine type protease.

APPLICATIONS:

  • Used extensively in protein sequencing
  • Tissue culture work
  • Tissue dissociation
  • Microbiological media
ANALYSIS SPECIFICATION TYPICAL
ASH (RESIDUE UPON IGNITION) Maximum 2.5% 0.2%
MOISTURE (LOSS ON DRYING) Maximum 5% 3.0%
STANDARD PLATE COUNT Maximum 1000 CFU/gram <1000 CFU/gram
YEAST/MOLD Maximum 10 CFU/gram 6 CFU/gram
ESCHERICHIA COLI Not Detected in 25 grams Not Detected
PSEUDOMONUS AERUGINOSA Not Detected in 25 grams Not Detected
SALMONELLA Not Detected in 25 grams Not Detected
STAPHYLOCOCCUS AUREUS < 10 CFU/GRAM Not Detected
PARVOVIRUS Negative Negative
ENDOTOXIN (GEL CLOT) <10 EU/mg <1 EU/mg
CHYMOTRYPSIN ACTIVITY MAXIMUM 75 UNITS/ mg 47 UNITS/mg
TRYPSIN ACTIVITY MAXIMUM 250 UNITS/ mg MINIMUM 250 UNITS/ mg

CHYMOTRYPSIN:: THE AMOUNT OF ENZYME CAUSING A DECREASE IN ABSORBANCE AT 237 nm
of 0.00075 PER MINIUTE AT 25C.
TRYPSIN:  THE AMOUNT OF ENZYME CAUSING A DECREASE IN ABSORBANCE AT 253 nm of 0.003 PER MINIUTE AT 25C.
PORCINE PARVOVIRUS: TESTED VIA PCR OR 9CFR METHOD

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