TRYPSIN -PORCINE 1:250-LOW ENDOTOXIN
T-88

DESCRIPTION:
Trypsin T-88 is a proteolytic enzyme that is a off white to beige colored powder. T-88 is extracted from USDA certified porcine pancreas glands. It is classified as a Serine type protease.

APPLICATIONS:

  • Used extensively in protein sequencing
  • Tissue culture work
  • Tissue dissociation
  • Microbiological media
ANALYSIS SPECIFICATION TYPICAL
ASH (RESIDUE UPON IGNITION) Maximum 2.5% 0.88%
MOISTURE (LOSS ON DRYING) Maximum 5% 2.5 %
STANDARD PLATE COUNT Maximum 1000 CFU/gram <1000 CFU/gram
YEAST/MOLD Maximum 10 CFU/gram 6 CFU/gram
ESCHERICHIA COLI Not Detected in 25 grams Not Detected
PSEUDOMONUS AERUGINOSA Not Detected in 25 grams Not Detected
SALMONELLA Not Detected in 25 grams Not Detected
STAPHYLOCOCCUS AUREUS Not Detected in 11 grams Not Detected
PARVOVIRUS ( PCR ) Negative Negative
ENDOTOXIN (GEL CLOT) <10 EU/mg 1 EU/mg
CHYMOTRYPSIN ACTIVITY 75-180 units/mg 120 units/mg
TRYPSIN ACTIVITY > 225 units/mg 400 units/mg
PROTEASE 225-275 UNIT/mg 240 units/mg

CHYMOTRYPSIN: THE AMOUNT OF ENZYME CAUSING A DECREASE IN ABSORBANCE
AT 237 Nm of 0.00075 PER MINIUTE AT 25C.
TRYPSIN: THE AMOUNT OF ENZYME CAUSING A DECREASE IN ABSORBANCE AT 253 nm of 0.003 PER MINIUTE AT 25C.
IRRADIATION: MINIMUM DELIVERED DOSE 25 kGy
PORCINE PARVOVIRUS: TESTED VIA PCR OR 9CFR METHOD

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